#REGENERON STEM CELL SERIAL#

(I) Apoe −/− mouse: extensive plaques (arrows n = 3).

(H) Apoe −/− rat little to no plaques (n = 3). (G–I) Oil red staining of aortas to detect atherosclerotic plaques. 2 WT and 3 Apoe +/− animals were assayed. (B) PCR gel confirming targeting of the Apoe locus (arrow expected band sizes are in Table S7). The entire coding sequence of the rat Apoe gene is replaced by a promoterless lacZ reporter and a neomycin selection cassette. Phenotyping Apoe KO rats (A) Design of the Apoe KO targeting vector. Using these methods, we have successfully replaced entire rat genes spanning up to 101 kb with the human ortholog.ĭisease models embryonic stem cells genetic modification germline transmission rat.Ĭopyright © 2022 The Author(s).

#REGENERON STEM CELL SERIAL#

These rESC lines maintain their potential for germline transmission after serial targeting with bacterial artificial chromosome (BAC)-based targeting vectors, and CRISPR-Cas9 cutting can increase targeting efficiency. We report here the generation of germline-competent rESC lines from several rat strains. CRISPR-based gene editing in zygotes is widely used in rats but is limited by the difficulty of inserting or replacing DNA sequences larger than about 10 kb.

Rat ESCs (rESCs) were first reported over 10 years ago, yet they are not as frequently used as mESCs. Rats were more frequently used than mice to model human disease before mouse embryonic stem cells (mESCs) revolutionized genetic engineering in mice.

Regeneron stem cell